Determination of Seven Water-soluble Vitamins in Tarhana, a Traditional Turkish Cereal Food, by High-performance Liquid Chromatography

نویسنده

  • R. Ekinci
چکیده

A reversed-phase high-performance liquid chromatographic procedure has been developed for determination of water-soluble vitamins (ascorbic acid, niacin, panthothenic acid (vitamin B5), pyridoxine (vitamin B6), thiamine (vitamin B1), folic acid, and riboflavin (vitamin B2)) in tarhana samples, a traditional Turkish cereal food. Sample-treatment entails SPE with Sep-Pak C18 (500-mg) cartridges. The water-soluble vitamins were analyzed by HPLC on a Discovery C-18 150 mm × 4.6 mm column with 0.1 mol L KH2PO4 (pH 7.0)–methanol, 90:10, as mobile phase (0.7 mL min) in isocratic mode. Identification of compounds was achieved by comparing their retention times and UV spectra with those of standards stored in a data bank. The detection limits ranged from 0.1 to 0.5 mg L. The accuracy of the method was tested by measuring average recovery; values ranged between 96.51 and 99.40%.

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تاریخ انتشار 2005